--- type: "WebPage" title: "Lipid formulation analysis by HPLC-CAD (Corona detector) and HPLC-MS/MS in the laboratory" description: "You want to analyse lipid formulations using HPLC-CAD (corona detector) and HPLC-MS/MS What is lipid analysis? skip render: ucaddon_section_textes_image Why control the quality of your lipid formulations? The biopharmaceutical industry is one of the most demanding when it comes to..." resource: "https://filab.fr/en/sectors-of-activity/biopharmaceutical/analysis-lipid-formulations/" tags: ["EN", "pll_672b9117f2fea"] timestamp: "2026-05-26T08:12:32Z" published: "2024-11-07T07:58:41Z" language: "en" author: "Laure Durieux" --- # Lipid formulation analysis by HPLC-CAD (Corona detector) and HPLC-MS/MS in the laboratory ## You want to analyse lipid formulations using HPLC-CAD (corona detector) and HPLC-MS/MS ### What is lipid analysis? Lipid analysis is the identification, quantification, and characterization of lipid molecules in pharmaceutical and biopharmaceutical formulations. Lipids - including triglycerides, phospholipids, and cholesterol derivatives - are critical excipients and active components in drug delivery systems, liposomal formulations, and emulsions. Accurate lipid analysis ensures batch consistency, stability, and regulatory compliance. ### Why control the quality of your lipid formulations? The [**biopharmaceutical industry**](https://filab.fr/en/sectors-of-activity/biopharmaceutical.md) is one of the most demanding when it comes to regulations. Manufacturers of biopharmaceutical formulations face particularly strict quality standards. The regulations surrounding the **quality of biopharmaceutical formulations** are not limited to the production phase; they extend to every stage of the product life cycle, including development, clinical trials and marketing. Quality control tests are defined by official pharmacopoeias such as the USP (United States Pharmacopeia) and the European Pharmacopoeia, ensuring that each formulation meets the highest international requirements. Non-compliance of a biopharmaceutical formulation, whether due to the presence of impurities or unexpected interactions between the active ingredient and excipients, can lead to refusal of marketing authorisation, or even withdrawal of the product already on the market. ## The FILAB laboratory can help you analyse lipid formulations using HPLC-CAD (corona detector) and HPLC-MS/MS Services ### The advantages of HPLC-CAD for the analysis of lipid formulations Universal detection: DAC can analyse lipids without chromophores, such as triglycerides or phospholipids, which is not possible with conventional UV detection. Accurate quantification: The linearity and sensitivity of DAC provide accurate quantification, essential for the analysis of complex formulations. High resolution: HPLC ensures efficient separation of the different lipid components, enabling precise characterisation of the formulation. Versatility: This technique is applicable in several industrial sectors (cosmetics, pharmaceuticals) where lipids play a crucial role in the stability and efficacy of formulations. Services ### The advantages of HPLC-MS/MS for the analysis of lipid formulations It enables precise qualitative and quantitative analysis, thanks to the fine identification of lipid species and quantification using specific ion transitions. This technique is suitable for complex matrices, such as formulations containing several excipients, active ingredients or lipid nanoparticles, without major interference. It is fast and does not require derivatisation, simplifying sample preparation and enabling a complete lipid profile to be obtained in a single analysis. ### Why choose FILAB for your lipid formulation analyses? For over 30 years, the FILAB laboratory has had the experience and specific [analytical equipment](https://filab.fr/en/our-technical-resources.md) to support companies in the analysis and assay of lipid formulations, through **tailor-made assistance**. From analysis to R&D, the FILAB laboratory offers expertise in the analysis and assay of lipid formulations using HPLC-CAD (corona detector). ## Our analysis services for lipid formulations Fatty acid analysis Identification and quantification of lipid types and molecular structures Analysis of chemical bonds and identification of functional groups in lipids Observation of the structure and distribution of lipids in formulations Study of sample weight loss as a function of temperature Analysis of triglycerides, phospholipids and other complex lipids Precise identification and quantitative analysis of lipids after chromatographic separation Study of the thermal properties of lipid formulations Determining the crystalline structures of lipids ## Our other technical resources [GC-MS](https://filab.fr/en/our-technical-resources/laboratory-analysis-gcms.md), [GC-MSMS ](https://filab.fr/en/our-technical-resources/gc-msms-analysis-laboratory.md) [SEM-EDX](https://filab.fr/en/our-technical-resources/laboratory-analysis-sem-scanning-electron-microscopy.md) [Mass Spectrometry](https://filab.fr/en/our-technical-resources/laboratory-analysis-mass-spectrometry.md) [DSC](https://filab.fr/en/our-technical-resources/laboratory-analysis-dsc-calorimetric.md) [TGA](https://filab.fr/en/our-technical-resources/tga-tga-ftir-and-tga-ms-analysis-at-filab-laboratory.md) [HPLC - MS, HPLC-MSMS](https://filab.fr/en/our-technical-resources/laboratory-analysis-uplc/laboratory-analysis-hplc-lcms.md) [RMN ](https://filab.fr/en/our-technical-resources/laboratory-analysis-nmr.md) [FTIR](https://filab.fr/en/our-technical-resources/laboratory-analysis-ftir-spectroscopy.md) [XRD](https://filab.fr/en/our-technical-resources/x-ray-crystallography-laboratory-xrd.md) ### Why choose HPLC-Corona to analyze lipid formulations? The CAD Corona is a detector that can be used for [LC, HPLC](https://filab.fr/en/our-technical-resources/altis-lc-msms-analysis.md) and [UPLC](https://filab.fr/en/our-technical-resources/laboratory-analysis-uplc.md) analysis. It generates a signal as a function of the molecules charged (by a gas after desolvation) and produces a graph of the signal intensity as a function of time. This detector is universal and its response is independent of the chemical structure of the targeted molecule, except that the latter must be non-volatile or semi-volatile. Analysis by LC-Corona enables both **detection and quantification** of the molecules analysed. It therefore works with a wide variety of molecules and offers great versatility in analytical chemistry. LC-Corona analysis can therefore be applied to a wide variety of sectors, including biopharmaceuticals and pharmaceuticals.   ![](https://filab.fr/wp-content/uploads/2025/03/HPLC-UV-CAD-min-1-1024x768.png) ## Our FAQ What is HPLC-CAD detection? Corona CAD is a universal detector that generates a signal based on charged molecules after desolvation. It is ideal for non-volatile and semi-volatile compounds such as lipids. Why is lipid analysis important in biopharmaceuticals? Lipids are critical excipients and active components in drug delivery systems. Accurate analysis ensures batch consistency, stability, and compliance with USP and European Pharmacopoeia standards. Which lipid classes can FILAB analyze? Triglycerides, phospholipids, cholesterol derivatives, and other complex lipids in formulations. What is the difference between HPLC-CAD and HPLC-MS/MS for lipid analysis? HPLC-CAD provides universal quantitative detection independent of chemical structure. HPLC-MS/MS adds structural identification and molecular species profiling capabilities. How to get a quote from FILAB? To obtain a quote, you can contact our team via our contact form, by phone, or by email. Simply tell us your requirements (type of material, desired analysis, applicable standards, urgency, quantity of samples, etc.). We will then send you a personalized technical and pricing proposal within 24-48 hours. What is the typical duration of the analysis? Turnaround times vary depending on the nature of the analysis and the complexity of the expert assessment project. However, FILAB is committed to providing fast turnaround times tailored to your industrial constraints and urgent needs.