---
type: "WebPage"
title: "Laboratory for analysis and measurement of circular dichroism (CD)"
description: "You wish to perform the analysis and measurement of circular dichroism (CD) Structural analysis of biomolecules and chiral materials FILAB laboratory, an expert in biopharmaceutical analysis, offers its clients its expertise in circular dichroism (CD) measurement to characterize the secondary..."
resource: "https://filab.fr/en/sectors-of-activity/biopharmaceutical/measurement-circular-dichroism-cd/"
tags: ["EN", "pll_691b389279867", "Anaïs DECAUX"]
timestamp: "2026-03-12T08:16:07Z"
published: "2025-11-17T15:30:24Z"
language: "en"
author: "Laure Durieux"
---

# Laboratory for analysis and measurement of circular dichroism (CD)

## You wish to perform the analysis and measurement of circular dichroism (CD)

### Structural analysis of biomolecules and chiral materials

FILAB laboratory, an expert in [biopharmaceutical analysis](https://filab.fr/en/sectors-of-activity/biopharmaceutical.md), offers its clients its expertise in **circular dichroism (CD)** measurement to characterize the **secondary structure** of [proteins](https://filab.fr/en/sectors-of-activity/biopharmaceutical/biotherapeutic-recombinant-protein-characterization/protein-analysis.md) and [peptides](https://filab.fr/en/sectors-of-activity/biopharmaceutical/biotherapeutic-recombinant-protein-characterization/peptide-analysis-biopharmaceutical-services.md), monitor the conformation of biotherapies, [control the chirality](https://filab.fr/en/our-services/our-analysis-services/chemical-analysis-laboratory/chiral-analysis.md) of materials and [evaluate the stability of your formulations](https://filab.fr/en/sectors-of-activity/health-pharmaceutical-cosmetics/stability-testing-medications-and-active-ingredients.md) under near-real conditions.

### Our services and study formats for Circular Dichroism (CD)

  Standard characterization (far UV CD)
##

 Single spectrum or multiple repetitions Deconvolution (α helices / β sheets / coil) Summary report   Comparability study
##

 Comparison between batches/formulations Similarity indicators (Δ ellipticity, spectral overlap, internal metrics) Formulation/Stability Recommendations   Thermal stability and/or pH
##

 Temperature ramp (10→95°C) with monitoring of the Tm Effect of pH, salts and excipients Reversibility study (cooling)   Interaction & screening
##

 Spectra before/after ligand (small molecule, ion, cofactor) Short kinetic energy if necessary Analysis of conformational changes
### Our other services in biopharmaceuticals

## identity and structure

[Peptide mapping](https://filab.fr/en/sectors-of-activity/biopharmaceutical/biotherapeutic-recombinant-protein-characterization/peptide-analysis-biopharmaceutical-services.md)

[Determination of intact mass](https://filab.fr/en/sectors-of-activity/biopharmaceutical/intact-mass-analysis-laboratory.md)

[Glycosylation:](https://filab.fr/en/sectors-of-activity/biopharmaceutical/glycosylation-analysis.md) elucidation of carbohydrate structure, monosaccharide composition, identification of glycosylation sites

[De novo sequencing](https://filab.fr/en/sectors-of-activity/biopharmaceutical/biotherapeutic-recombinant-protein-characterization/de-novo-protein-sequencing.md)

[Analysis and localisation of disulfide bridge](https://filab.fr/en/sectors-of-activity/biopharmaceutical/disulfide-bridge-analysis.md)

## Our complementary analytical techniques

 [### FTIR

To confirm the secondary structure](https://filab.fr/en/our-technical-resources/laboratory-analysis-ftir-spectroscopy.md) [### DSC

To measure thermal transitions](https://filab.fr/en/our-technical-resources/laboratory-analysis-dsc-calorimetric.md) [### SEC

To evaluate molar mass and aggregation](https://filab.fr/en/our-technical-resources/laboratory-analysis-gpc-sec.md) [### LC-MS

To analyze the integrity and composition of biomolecules](https://filab.fr/en/sectors-of-activity/biopharmaceutical/biotherapeutic-recombinant-protein-characterization/peptide-analysis-laboratory-by-lc-ms.md)
## The FILAB laboratory performs the analysis and measurement of circular dichroism (CD)

### Why perform a Circular Dichroism (CD) analysis?

The CD is an essential tool for:

Study the **secondary structure** (α helices, β sheets, disordered conformations)

Compare batches, formulations, or storage conditions

Checking structural integrity after a process change

Checking the**conformational stability** of a protein

Follow a **thermal or chemical denaturation**

Detecting subtle differences between two versions of the same biomaterial (biosimilars, variants, mutations…)

### What FILAB offers you for your Circular Dichroism measurements CD

**Expert support**: assistance with experimental design (spectral ranges, temperature, buffer, concentration)

**Stability studies**

**Fast and robust measurements**: spectra in the far UV (190–260 nm) and/or near UV (260–320 nm)

**Confidentiality and quality**: project management, traceability, and services under standards adapted to your sector

## FAQ

 Does Circular Dichroism (CD) replace DSC or NMR?
No. Circular Dichroism CD is fast and sensitive to variations in secondary structure; it is complementary to DSC (thermodynamics) and NMR (fine structure, local interactions).

 How far can we go below 200nm?
This depends on the **transparency of the buffer** and the optical path. We optimize the conditions to maximize the useful information..

 Can studies be conducted under real-world conditions (salt, excipients)?
Yes, subject to optical compatibility. We offer **alternative formulations** when necessary.

 What are the areas of application of Circular Dichroism (CD)?
- **Biopharmaceuticals**: antibodies, fragments, therapeutic peptides, enzymes, vaccines, biosimilars
- **Biotechnology and diagnostics**: recombinant proteins, media, reagents
- **Cosmetics and dermo-cosmetics**: functional peptides, skincare proteins, formulation stability