Objectives
Understanding the operating principles of LC/MSMS and LC/MS
Meet increasingly stringent regulatory requirements and constraints
Acquire theoretical and practical skills in this area
Know how to acquire and reprocess data
Pedagogical resources
- Screening of training material
- Handover of training material
- Training led by experts in the techniques required
Target audience
Technicians or engineers in charge of research & innovation, process & industrialisation, production & maintenance, quality control, health, safety & environment, purchasing & supplies …
Program
For more information on the program, please contact us
Information updated on 10.10.2024
Practical information
- In-company training
- Questionnaire at the beginning and end of the course (Quizzes)
- Attendance sheet
- Duration: 2 days
- PRM and PSH accessibility
- Adaptable to customer needs
- Price on quotation
- No pre-requisites required for this course
- A certificate of completion will be issued at the end of the course.
Our training courses are accessible to people with disabilities. We will offer you solutions for adaptations (e.g. training aids, pace of training, teaching support, etc.) and adjustments to suit your needs.
Your teams need training in LC/MSMS et LC/MS...
What is MS mass spectrometry?
Thanks to technical advances in recent years, mass spectrometry is a method that is often used to meet these criteria of precision and sensitivity. It provides both quantitative (elemental content) and qualitative (nature) data, enabling overall analysis times to be reduced.
Combined with LC (or HPLC), MS offers better detection quality and can analyse unknown peaks or very low concentration degradation products of any type (organic or inorganic).
At FILAB, we have a dual MS/MS detection system for analysing very low concentrations.
Why train in LC-MSMS and LC-MS?
There are many applications for LC/MS/MS and LC/MS analysis. This course will enable you to :
Carry out precise quantitative and qualitative analysis to identify and quantify compounds in complex matrices.
Detect and characterize contaminants, impurities or traces of substances in various sectors (pharmaceutical, agri-food, environment, etc.).
Anticipating and resolving technical faults to maximise the efficiency and performance of your equipment.
Optimise the development of analytical methods tailored to your laboratory's requirements.
Improve the sensitivity and specificity of your analysis for reliable, reproducible results.
Training in LC-MS/MS and LC-MS means acquiring the essential skills needed to make full use of this technology in the laboratory.
Why take an LC MS training course?
Liquid chromatography coupled with mass spectrometry (LC/MS) is a common technique in physico-chemical analysis and bioanalysis. Thanks to technological advances, its use has been extended to a wide range of applications, both for quantitative analysis (dosing) and qualitative analysis (structural identification).
However, to fully exploit the potential of LC/MS(/MS) requires a solid theoretical understanding and in-depth mastery of its fundamental mechanisms. The training provided by FILAB aims to provide the essential knowledge to start or perfect your LC/MS or LC-MS/MS analysis.
FAQ
This course aims to provide an in-depth understanding of the operating principles of LC-MS/MS and LC-MS, while ensuring mastery of the ever-changing regulatory requirements. It provides the theoretical and practical skills essential for optimising the use of these analytical techniques. Participants will also learn how to acquire and reprocess data, thereby guaranteeing reliable analyses that comply with quality standards.
This course is designed for engineers and laboratory technicians who are new to LC/MS and want to master the fundamentals and improve their skills.
On completion of the course, participants will be able to:
- Understand the theoretical and practical principles of LC/MS.
- Develop analytical methods adapted to the needs of the laboratory.
- Identify and correct common problems to ensure reliable and reproducible analyses.
No, this course is also designed for beginners in LC/MS. A general knowledge of analytical techniques is a plus, but not essential. What's more, our courses can be tailored to suit your needs.
It combines the two aspects:
Theory to understand the fundamental principles.
Practice, with case studies and practical solutions to optimise your analysis.
By mastering good LC/MS practice, participants will be able to:
- Develop reliable and reproducible methods.
- Minimise errors and time wasted due to technical anomalies.
- Increase the accuracy and speed of analysis.
Liquid chromatography coupled to mass spectrometry (LC-MS) and its tandem version (LC-MS/MS) may be referred to by several other names depending on the context and field of application:
🔹 HPLC-MS (High-Performance Liquid Chromatography-Mass Spectrometry) - Used when high performance liquid chromatography is specified.
🔹 LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry) - Also known as MS², denoting tandem analysis for greater selectivity and sensitivity.
🔹 TOF-SIMS (Time-of-Flight Mass Spectrometry) - When a Time of Flight mass analyser is used.
🔹 HRMS (High-Resolution Mass Spectrometry) - When high-resolution mass spectrometry is used, often in combination with LC.
🔹 QTOF-MS (Quadrupole Time-of-Flight Mass Spectrometry) - Designating a configuration where a quadrupole is coupled to a TOF analyser.
These different designations vary depending on the equipment and the specific applications (pharmaceutical, environmental, agri-food, bioanalysis, etc.).
TO GO FURTHER
Optimising sensitivity relies on several factors:
✔ Choice of mobile phase: Use volatile buffers such as ammonium acetate or ammonium formate to avoid ionisation suppression.
✔ Optimisation of source parameters: Adjust desolvation temperature, capillary voltage and nebulisation pressure to maximise ionisation.
✔ Ionisation mode adjustment (ESI/APCI): Select the appropriate ionisation source based on analyte polarity and volatility.
✔ Optimising the signal-to-noise ratio: Work on sample dilution and elimination of matrix interference via SPE or isotopic dilution.
Matrix effects, which can lead to signal suppression or enhancement, can be reduced by:
✔ Using isotopic internal standards to compensate for ionisation variations.
✔ Optimising sample preparation: solid phase extraction (SPE) or efficient filtration.
✔ Modifying mobile phase composition: buffering and adjusting pH to improve separation.
✔ Working on sample dilution to reduce the co-eluting effects of impurities.
The choice of fragmentation mode (CID - Collision-Induced Dissociation, HCD - Higher-energy Collisional Dissociation) directly influences:
✔ The specificity of the fragments: Certain modes generate more specific ions for identification.
✔ Sensitivity: Some fragments have a better response depending on the collision energy applied.
✔ Resolution: Too high a setting can reduce ion transmission and decrease sensitivity.
In the pharmaceutical industry, LC-MS/MS ensures quality control by enabling:
✔ Precise dosing of active ingredients and detection of impurities (ICH Q3A/B standards).
✔ Analysis of degradation products to guarantee drug stability.
✔ Bioanalysis in pharmacokinetics, essential for bioavailability studies.
