Laboratory analysis by LC-CAD

You wish to carry out an analysis by LC-CAD in laboratory

Why perform an analysis by LC-CAD?

First of all, the acronym LC-CAD stands for Liquid Chromatography with Charged Aerosol Detector. 

The associated apparatus is used to detect molecules of interest in mixtures. It is used in combination with a high performance liquid chromatography (HPLC) system that performs the separation of molecules in solution.

The CAD is an HPLC detector that generates a signal based on the charges from the molecules and produces a graph of the signal intensity versus time. This detector works with a wide variety of molecules since it does not need a specific structure on the analyte to detect it. It is therefore versatile in analytical chemistry. The analysis by LC-CAD can thus be applied to a great variety of sectors (pharmaceutical, etc…)

Indeed, the analysis by LC-CAD allows the detection, but also the quantification of the analyzed molecules.

FILAB laboratory can assist you in your LC-CAD analysis needs

Through our three levels of services: analysis, expertise and R&D support, FILAB assists companies from all sectors in their LC-CAD analysis needs. FILAB provides its customers with the know-how and experience of its team, as well as a 2100m² analytical park equipped with state-of-the-art equipment.

The positive aspects of FILAB

  • A highly qualified team

  • Responsiveness in responding to and processing requests

  • A complete analytical park of 2100m²

  • Tailor-made support

Diving into the world of analytical techniques, it's important to understand the difference between Liquid Chromatography (LC) and Charged Aerosol Detector (CAD). LC refers to the separation of molecules in a solution, based on their size or charge. On the other hand, CAD is a cutting-edge instrument that enables a comprehensive quantitative and qualitative analysis of the sample, particularly in the pharmaceutical field of application.


Rather than pitting LC vs CAD, these two techniques are actually meant to be combined. By harnessing the power of both LC and CAD, researchers can detect molecules of interest with this remarkable device.

Liquid chromatography with charged aerosol detection (LC-CAD) offers multiple advantages for analytical testing. One key advantage is the elimination of sample pre-treatment, saving time and effort in the testing process.

Additionally, LC-CAD provides enhanced sensitivity and broader identification capabilities for various types of molecules. This means more accurate and reliable results when analyzing samples.


In this method, the sample is diluted in a solvent, known as the mobile phase, and then separated by the solid-type stationary phase in a column. Each molecule moves at its own specific speed, facilitating the identification of analytes with the help of charged aerosol detection.


By using LC-CAD analysis, researchers and technicians can achieve precise and efficient identification of molecular compounds.This technique is fundamental in various industries and research fields that rely on accurate sample analysis.

Charged Aerosol Detection (CAD) is a powerful detector that analyzes the charge of molecules separated by Liquid Chromatography. By creating a graph that displays the intensity of the signal over time, CAD allows for precise and accurate measurements.


CAD operates through three main stages: nebulization, charging, and detection. Nebulization uses evaporation technology to eliminate solvents and form droplets.These droplets then collide with gas in an ionization chamber, resulting in a positive charge on the particles. The size of the particles determines their charge capacity. To analyze the signal emitted, a highly sensitive electrometer is used. This electrometer takes into account the quantity of analytes, which is directly proportional to the signal intensity. In other words, the more analytes present, the stronger the signal.


In summary, CAD works by converting analytes into a detectable signal, allowing for precise and reliable analysis of molecules.

Thomas ROUSSEAU Scientific and Technical Director
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