Chromatographie LC/GC : troubleshooting

How can troubleshooting help you overcome unexpected analytical challenges?

Liquid Chromatography (LC, HPLC/UPLC) and Gas Chromatography (GC) are essential analytical techniques for analytical, research, and quality control laboratories. They enable the separation, identification, and quantification of compounds in complex matrices with high precision. However, even when methods are carefully developed and validated, unexpected analytical challenges can arise, disrupting your routine analyses. Implementing troubleshooting to diagnose a problem encountered during production or method implementation is therefore an effective solution.

Common analytical challenges in LC/GC chromatography

Even the most well-established methods can show signs of drift or unexpected anomalies. Here are some examples of disruptions that can be resolved with a methodical approach and in-depth analysis called troubleshooting:

Peak splitting: The appearance of double peaks may be due to poor column alignment, contamination, or moving phase problems.

Peak shoulder: This effect may indicate a problem with the interaction between the sample and the stationary phase or an imbalance in the mobile phase.

Appearance of new peaks: an unknown peak may indicate contamination of the sample, the column, or even degradation of the analyte.

Variable retention times: Fluctuations in retention times can be caused by changes in pressure, temperature variations, or changes in the composition of the mobile phase.

Here are some examples of disruptions that can be resolved with a methodical approach and in-depth analysis called troubleshooting.

The difficulties of validation and method transfer

In addition to the problems encountered during routine analyses, the validation and transfer of methods from one laboratory to another often pose challenges. Validation involves demonstrating that the method meets criteria for precision, accuracy, specificity, and robustness. During method transfer, factors such as equipment configuration, solvent quality, or technician training can lead to inconsistent results.

Troubleshooting: optimization solutions and personalized support

If you are facing these problems, it is essential to understand the source of the anomalies in order to remedy them effectively. Here is how specialized support (troubleshooting) can help you optimize your methods and overcome these challenges:

In-depth analysis of existing methods: a comprehensive diagnosis helps identify the source of problems and suggest specific adjustments. This may include checking instrument settings, column condition, solvent quality, and experimental conditions.

Method optimization: Whether you need to adjust the mobile phase, choose a new column, or modify elution conditions, experts can guide you in redesigning your method to improve resolution and stability.

Co-development of methods: working in collaboration with an experienced laboratory allows methods to be developed or adjusted, taking into account the specific characteristics of your samples and the desired performance.

Transfer and method validation: support in method transfer ensures that the method is reliably reproduced in a new environment by identifying and adjusting factors that could influence the results.

Why perform troubleshooting with a laboratory that specializes in chromatography?

At Filab, we understand the importance of maintaining reliable and reproducible analyses. Our teams of LC and GC chromatography experts can help you identify and resolve your analytical issues, using advanced techniques and offering tailor-made solutions adapted to your needs. Thanks to troubleshooting and our expertise, we enable you to overcome your unexpected analytical challenges.

To conclude:

Analytical challenges in LC/GC chromatography, although common, can be overcome with a methodical approach and expert guidance. Whether you are experiencing abnormal peaks, unstable retention times, or difficulty validating or transferring a method, it is possible to find suitable solutions and continue your analyses with confidence and efficiency.

We are available to answer any questions you may have.

LC/GC chromatography: troubleshooting and optimization to overcome your unexpected analytical challenges