Laboratory analysis using High Performance Liquid Chromatography (HPLC)
Your needs: analyze active substances or impurities in your products by High-Performance Liquid Chromatography (LC) and High-Performance Liquid Chromatography (HPLC)
What is HPLC analysis?
High-Performance Liquid Chromatography (HPLC) is used to identify and quantify organic substances (active ingredients or contaminants) in various, sometimes complex, matrices: cosmetics, pharmaceuticals, natural ingredients, biological matrices…
HPLC analysis using liquid chromatography (HPLC) is an analytical technique used to separate, identify and quantify compounds in solution. In short, this method dissociates the molecules present on the basis of their physico-chemical characteristics.
Why use Liquid Chromatography (LC) analysis?
Liquid chromatography (LC) is an analytical technique widely used in chemistry laboratories for samples containing several compounds, such as plant extracts or biological samples, but also for the identification of contaminants or impurities.
LC can be used to analyze impurities in pharmaceutical products or contaminants in drinking water, for example. The results obtained with liquid chromatography are highly accurate and reliable, making it a preferred technique.
Our services for HPLC laboratory analysis
FILAB is a full-service laboratory for liquid chromatography (HPLC) analysis. Our HPLC analyses are carried out with precision and rigor, and we work closely with our customers to adapt our analytical methods to their specific needs.
Active ingredient analysis
Determination of regulated substances: nitrosamines, bisphenol, adhehydes
Our solutions: analysis by liquid chromatography (HPLC)
The know-how of our highly qualified teams enables us to offer you our expertise in the analysis of your materials using high-performance liquid chromatography. Our specialists will be with you from the very first step, through to the interpretation of the results, in order to offer you lasting corrective solutions.
The advantages of laboratory HPLC analysis
The advantages of laboratory HPLC analysis are numerous.
First and foremost, this technique offers high accuracy and sensitivity in molecule identification. It also allows great flexibility in terms of the types of samples and compounds tested. What’s more, HPLC laboratory analysis is often complemented by additional detection techniques, such as mass spectrometry, for more in-depth analysis. Finally, this technique is particularly useful in the pharmaceutical and food industries for guaranteeing product quality, safety and conformity.
Examples of materials subjected to HPLC analysis
The materials subjected to HPLC analysis are extremely varied, ranging from organic compounds to polymers and metals. Natural substances, such as amino acids, proteins and sugars, are often examined by HPLC. Drugs are also subject to HPLC analysis, as are impurities in pharmaceutical products.
In short, HPLC represents a key analytical method for efficiently examining a wide range of materials and substances.
Our Liquid Chromatography (LC) analysis by sector of activity...
Sectors of activity
Our detectors: precise technical resources adapted to your sector
FILAB offers a wide range of detectors for high-performance liquid chromatography (HPLC) analysis. The choice of detectors depends on the nature of the samples, the objectives of the analysis and the sensitivity required.
Commonly used HPLC detectors include :
FAQ
There are two commonly used types of chromatography:
- liquid chromatography (LC)
- high-performance liquid chromatography (HPLC).
These two techniques differ in the characteristics of their stationary and mobile phases. HPLC uses finer stationary phases and high-throughput pumps for better molecule resolution, whereas conventional liquid chromatography can be carried out at constant throughput and with finer stationary phases. In all cases, the aim is to achieve a clean separation of the sample components, enabling precise analysis of each substance present. Ultimately, this translates into higher resolution and faster separation of components in a sample with HPLC.
Physico-chemical properties such as acidity, basicity, polarity, solubility and molecular size are important for the separation of compounds, and are therefore essential for HPLC. These properties determine the behavior of compounds on the chromatographic column, and thus the identification of sample components.
HPLC can be applied to :
- Aqueous or organic solutions
- Raw materials
- Finished products (creams, tablets, liquids, etc.)
- Synthesis intermediates
- Complex samples (biological, environmental...)
The filab laboratory can develop and validate HPLC methods according to the specific requirements of your product, in compliance with ICH, Pharmacopoeia and other standards.
Thanks to optimized methods and high-performance detectors (e.g. HPLC-MS), it is possible to quantify impurities down to a few ppm, or even ng/mL in some cases.
