Oligonucleotide and antisense oligonucleotide analysis laboratory (ASO)
Are you looking to analyse and characterise your oligonucleotides and antisense oligonucleotides (ASOs)?
Why analyse and characterise your oligonucleotides and antisense oligonucleotides (ASOs)?
The analysis and characterisation of oligonucleotides and ASOs are crucial steps in guaranteeing their quality, therapeutic efficacy and safety for clinical use. These synthetic molecules, designed to specifically target RNA sequences, are used in gene therapies and interventions for genetic diseases. Their development requires an in-depth understanding of their structure and physico-chemical properties.
What is oligonucleotide and antisense oligonucleotide analysis (ASO)?
The characterization of oligonucleotides includes sequence, purity and stability analyses. It also involves identifying impurities linked to synthesis and degradation, and validating chemical modifications (such as phosphorothioates or sugar modifications) that increase the stability of ASOs in biological environments.
Particular attention is being paid to stability and enzymatic degradation, two key factors in the efficacy of ASOs under biological conditions.
Our solutions for the analysis and characterization of oligonucleotides and ASO
Why choose FILAB?
The FILAB laboratory offers comprehensive analytical services tailored to the specific needs of oligonucleotides and ASOs. With our expertise in biotechnology, we support you in the development, validation, and quality control of your molecules in compliance with current regulations.
We provide customized solutions throughout the development process, from synthesis to production, focusing on critical quality attributes to ensure the therapeutic efficacy of ASOs.
Oligonucleotide and ASO analysis and characterization services
Structure and sequence characterization :
Sequence analysis by LC-MS/MS
Analysis of degraded forms (synthetic impurities)
Verification of chemical modifications (phosphorothioate, methylation, etc.)
Physicochemical properties characterization:
We provide precise data on the size, molecular weight, and stability of oligonucleotides and ASOs:
Molecular weight (LC-MS)
Enzymatic degradation kinetics
Chromatographic profile (HPLC)
Impurity identification and quantification :
Synthetic impurities (HPLC, LC-MS)
Unwanted chemical modifications
We also perform compliance analyses according to ICH and USP standards, ensuring the safety of manufacturing processes. Our services include the quantification of elemental impurities and specific tests to evaluate extractables and leachables (E&L) during stability testing.
With rigorous characterization of your oligonucleotides and ASOs, you ensure not only the safety and efficacy of your products but also their regulatory compliance, guaranteeing their therapeutic success.
