Oligonucleotide and antisense oligonucleotide analysis laboratory (ASO)
Oligonucleotide and ASO analysis and characterization services
What is oligonucleotide and antisense oligonucleotide analysis (ASO)?
The characterization of oligonucleotides includes sequence, purity and stability analyses. It also involves identifying impurities linked to synthesis and degradation, and validating chemical modifications (such as phosphorothioates or sugar modifications) that increase the stability of ASOs in biological environments.
Particular attention is being paid to stability and enzymatic degradation, two key factors in the efficacy of ASOs under biological conditions.
Structure and sequence characterization
Sequence analysis by LC-MS/MS
Analysis of degraded forms (synthetic impurities)
Verification of chemical modifications (phosphorothioate, methylation, etc.)
We provide precise data on the size, molecular weight, and stability of oligonucleotides and ASOs:
Physicochemical properties characterization
Molecular weight (LC-MS)
Enzymatic degradation kinetics
Chromatographic profile (HPLC)
Impurity identification and quantification
Synthetic impurities (HPLC, LC-MS)
Unwanted chemical modifications
Analysis of solvent residues and culture medium (cell culture): measurement of residual amino acids, vitamins and saccharides
We also perform compliance analyses according to ICH and USP standards, ensuring the safety of manufacturing processes. Our services include the quantification of elemental impurities and specific tests to evaluate extractables and leachables (E&L) during stability testing.
With rigorous characterization of your oligonucleotides and ASOs, you ensure not only the safety and efficacy of your products but also their regulatory compliance, guaranteeing their therapeutic success.
Our solutions for the analysis and characterization of oligonucleotides and ASO
Why choose FILAB for your oligonucleotide analysis?
The FILAB laboratory offers comprehensive analytical services tailored to the specific needs of oligonucleotides and ASOs. With our expertise in biotechnology, we support you in the development, validation, and quality control of your molecules in compliance with current regulations.
We provide customized solutions throughout the development process, from synthesis to production, focusing on critical quality attributes to ensure the therapeutic efficacy of ASOs.
Our FAQ
Oligonucleotide analysis is used to confirm sequence identity, assess purity, detect impurities and evaluate stability in synthetic DNA or RNA molecules used in gene therapy and antisense treatments.
ASO analysis typically involves LC-MS, LC-MS/MS, HPLC and stability studies to verify molecular weight, chemical modifications and impurity profiles.
Impurity profiling ensures patient safety and regulatory compliance by identifying truncated sequences, synthesis by-products and degradation products.
Oligonucleotide testing supports compliance with ICH and USP guidelines by validating purity, stability and elemental impurity levels.
Sequencing confirms nucleotide order, while oligonucleotide analysis evaluates purity, structure, modifications and physicochemical properties.
To receive a quotation for oligonucleotide analysis or ASO characterization, you can contact our laboratory through the online form, by email or by phone.
To ensure an accurate proposal, please provide details such as the type of oligonucleotide (DNA, RNA, ASO), development stage (R&D, preclinical, GMP), required analyses (LC-MS, impurity profiling, stability testing, elemental impurities, etc.), applicable regulatory framework, sample quantity and expected timelines.
Our scientific team will review your request and provide a tailored technical and financial proposal adapted to your project requirements.
Turnaround times for oligonucleotide analysis depend on the analytical scope, regulatory requirements and complexity of the characterization (sequence confirmation, impurity profiling, stability studies, etc.).
Our oligonucleotide analysis laboratory works closely with biopharmaceutical companies to define timelines aligned with development milestones and regulatory constraints. Accelerated testing strategies can be implemented when required to support critical project phases.
To obtain a quote, you can contact our team via our contact form, by phone, or by email.
Simply tell us your requirements (type of material, desired analysis, applicable standards, urgency, quantity of samples, etc.). We will then send you a personalized technical and pricing proposal within 24-48 hours.
Turnaround times vary depending on the nature of the analysis and the complexity of the expert assessment project.
However, FILAB is committed to providing fast turnaround times tailored to your industrial constraints and urgent needs.
The analysis and characterisation of oligonucleotides and ASOs are crucial steps in guaranteeing their quality, therapeutic efficacy and safety for clinical use. These synthetic molecules, designed to specifically target RNA sequences, are used in gene therapies and interventions for genetic diseases. Their development requires an in-depth understanding of their structure and physico-chemical properties.
