Analysis by LC-Orbitrap in the laboratory
Do you need LC-Orbitrap analysis?
What is Orbitrap mass spectrometry?
Orbitrap mass spectrometry is an ultra-precise analysis technique that measures the exact mass of molecules present in a sample. It is used to identify, characterize and quantify even trace compounds.
This technology is based on an “Orbitrap” type analyzer, capable of delivering very high resolution and exceptional mass accuracy, often to within a few ppm (parts per million). It is generally coupled with upstream liquid chromatography (LC), hence the name LC-Orbitrap.
Orbitrap LC-MS: ultra-sensitive technology for identifying complex compounds
Orbitrap LC-MS mass spectrometry is a cutting-edge technique that combines liquid chromatography (LC) to separate compounds in a sample with an Orbitrap detector offering very high resolution and mass accuracy.
It can be used to identify, even in trace quantities, unknown or complex substances in a variety of industrial matrices (formulations, polymers, plant extracts, etc.). Ideal for detecting impurities, contaminants or migrating substances, this method is particularly valuable for the pharmaceutical, cosmetics, chemical and packaging industries.
How does Orbitrap mass spectrometry work?
Firstly, Orbitrap mass spectrometry allows the trapping of ions by an electrostatic field. These ions have a variable axial oscillation frequency depending on their mass to charge ratio, which allows them to be separated.
The Orbitrap consists of a central electrode and a cylindrical outer electrode. The central electrode, coaxial with the inner axis, traps the ions in an orbital motion and a mass spectrum can then be generated from the detector which converts the induced current image of the trapped ions into a frequency spectrum using the Fourier transform.
Therefore, Orbitrap mass spectrometry offers quantitative mass spectrometry coupled to liquid chromatography (LC-MS) with high mass resolution and accuracy (HRAM).
Why carry out Orbitrap lc ms analysis in the laboratory?
Analysis by LC-Orbitrap
Determine the molecular weight of an organic compound
Search for impurities at the root of a defect
Check the conformity of a raw material or finished product
Develop analytical methods specific to your products, including mass spectrometry
Our solutions: combining mass spectrometry with Orbitrap to provide you with reliable and accurate results
Why choose FILAB for LC-Orbitrap analysis?
At FILAB, we guarantee our customers accurate, reliable and fully documented analysis, carried out within a rigorous framework.
You benefit from personalized technical and regulatory support, tailored to your sector and your challenges.
Thanks to our full range of analytical equipment, we are able to combine LC-Orbitrap analysis with other complementary techniques (GC-MS, ICP, SEM, FTIR, etc.), to gain a deeper understanding of your issues.
The advantages of LC-Orbitrap analysis
Very high resolution to distinguish molecules very close in mass
High sensitivity for detection of trace substances
Non-targeted analysis possible for identification of unknown compounds
High mass accuracy, useful for elucidating the structure of molecules
Applications of Orbitrap LC MS analysis
Identification of unknown contaminants (stains, odours, residues on finished products)
The search for by-products or impurities in chemical or pharmaceutical formulations
Non-targeted analysis for R&D investigations or in-depth quality control
Profiling complex raw materials of plant or polymer origin
FAQ
The Orbitrap offers better resolution and greater accuracy than conventional mass spectrometers (quadrupole or ion trap type). It is particularly useful for identifying complex molecular structures, differentiating isomers or resolving very close masses in charged matrices.
All types of organic matrix: powders, liquids, polymers, plant extracts, thin films, solutions, cosmetics, etc.
It is particularly well suited to complex or multi-component samples, even in small quantities.
Yes, semi-quantitative or relative quantification is possible, especially for complex mixtures or screening. For precise regulatory dosages, it can be combined with other approaches (LC-MS/MS).
Thanks to its high resolution, the LC-Orbitrap is ideal for identifying unknown or unlisted organic compounds responsible for odours, colourations or visual defects. It can be used to analyse traces in complex matrices (cosmetics, polymers, formulations, etc.).
It can be used to detect trace impurities, including non-targeted impurities, which are often invisible in conventional LC-MS.
This is useful during pharmaceutical development, supplier qualification and process change phases.
Untargeted analysis with LC-Orbitrap can be used to propose a crude formula from a signal and to partially or fully elucidate the structure of a compound using MS/MS fragmentation and internal or external databases.
The LC-Orbitrap is ideally suited to the analysis of multi-component mixtures derived from plants, essential oils, bio-based polymers, etc. It enables complete profiling and differentiation between batches or suppliers, even in the absence of standards.
Yes, in particular to identify migrating substances in very low concentrations (phthalates, residual solvents, additives, etc.), as part of compatibility or regulatory compliance studies (pharmaceutical, cosmetics, food).
