As a manufacturer, you would like to carry out a peptide analysis using lc-ms
What is a peptide analysis?
A peptide analysis is a process that aims to characterise and identify peptides, molecules made up of chains of amino acids. These analyses are essential in the fields of biotechnology, pharmaceuticals, biomedical research and the agri-food industry. Peptides play a crucial role as biologically active molecules, often used as biomarkers, drugs or functional components.
Objectives of peptide analysis
Peptide analysis has several key objectives for research and industry. Firstly, they enable the structural characterization of peptides, by precisely determining the sequence of the amino acids that make them up. Secondly, they enable peptides to be identified by checking that they correspond to a reference or standard, thereby guaranteeing their authenticity.
Another major objective is quantification, which involves measuring the concentration or quantity of peptides present in a sample. Peptide analysis is also used to detect post-translational modifications, such as phosphorylation or glycosylation, which influence the function and biological properties of peptides. Finally, they play a crucial role in quality control, by assessing the purity and integrity of peptides, particularly in pharmaceutical applications where these parameters are decisive in guaranteeing the safety and efficacy of products.
The FILAB laboratory can help you with peptide analysis by lc-ms
Why carry out a Peptide analysis by lc-ms?
LC-MS analysis of peptides offers a number of advantages. Firstly, it offers high sensitivity, enabling the detection of peptides in very low concentrations, even in complex matrices. It is also distinguished by its high specificity, thanks to the effective combination of chromatography and spectrometry, which ensures accurate identification of peptides.
Another major advantage is its multiplexing capability, enabling several peptides to be analysed simultaneously in the same sample. In addition, this method provides detailed information, including comprehensive data on peptide structure, post-translational modifications and concentration.
Finally, LC-MS analysis is highly versatile, being suitable for a wide variety of samples, whether biological, pharmaceutical or environmental.
Applications of peptide analysis by lc-ms?
LC-MS analysis of peptides has a wide range of applications. In biomedical research, it is used to study bioactive peptides, biomarkers and antimicrobial peptides. In pharmaceutical development, it plays an essential role in the quality control of therapeutic peptides and biopharmaceutical products.
In proteomics, this technique enables the identification and characterization of proteins through their peptides derived after enzymatic digestion. Finally, in the agri-food sector, it is used to analyse functional peptides derived from food proteins, contributing to innovation in this field.
our other technical resources
Mass spectrometry (MS)
Mass-coupled Gas Chromatography (GC/MS)
Liquid chromatography HPLC-MS and HPLC-MS/MS (Orbitrap)
MALDI-TOF
LC-QTOF-MS
