Biopharmaceutical manufacturers, you wish to perform a fluorimeter analysis
Fluorimetry is an optical analysis technique based on the ability of certain molecules to emit light after excitation. It is particularly used in biopharmaceuticals for its sensitivity and selectivity.
Fluorimeter analysis: a key technique for the biopharmaceutical industry
Fluorimeter analysis is a reference technique for the detection, quantification, and monitoring of fluorescent compounds at very low concentrations. In the biopharmaceutical industry, it plays a central role in ensuring the safe development, production, and control of biomolecules, proteins, peptides, nucleic acids, and contaminants.
At the filab laboratory, this technique is implemented in a controlled environment, using highly sensitive equipment and expertise tailored to the regulatory and industrial constraints of the biopharmaceutical sector and the analysis of biological medicines.
Applications of fluorimetry analysis in biopharmaceuticals
Fluorimeter analysis is used at different stages of the biopharmaceutical product lifecycle:
Assay of proteins and biomolecules (BSA, antibodies, enzymes, peptides)
Purity control and detection of fluorescent impurities
Stability and degradation studies
Monitoring of bioproduction processes (fermentation, purification, formulation)
Residual DNA/RNA assay
Quality control in production
The FILAB laboratory assists manufacturers with fluorimeter analysis
When should you use a service laboratory specializing in fluorimeter analysis?
Manufacturers use the services of the FILAB laboratory, which has this technology, particularly to:
- meet regulatory or quality requirements
- investigate non-compliant results
- compare batches or formulations
- ensure the safety of analytical development
- outsource sensitive analyses not performed internally
Why use fluorimetry in biopharmaceuticals?
Fluorimetry is based on measuring the light emitted by a molecule after excitation. It is distinguished by:
- excellent sensitivity, often exceeding UV-visible absorbance;
- high selectivity, thanks to the precise choice of wavelengths;
- track detection, compatible with complex matrices.
These characteristics make it a technique particularly well-suited to the reliability, reproducibility, and traceability requirements specific to the biopharmaceutical sector.
FAQ
Yes. Thanks to its high selectivity and the precise choice of excitation and emission wavelengths, fluorimetry makes it possible to analyze complex matrices typical of the biopharmaceutical sector (culture media, formulations, excipients, buffers).
UV-visible spectroscopy measures light absorption, while fluorimetry measures light emission. This difference allows fluorimetry to offer better sensitivity, lower detection limits, and a better signal-to-noise ratio.
Yes. Labeling agents or fluorescent probes can be used to make non-naturally fluorescent molecules detectable, such as certain proteins, peptides, DNA, or contaminants.
Yes. Analyses can be carried out using validated methods, with full traceability of tests, meeting the quality and regulatory requirements of the biopharmaceutical sector.
