Peptide analysis in laboratory | Peptide mapping - Biopharmaceutical Services
You need to characterize peptides present in your matrices or finished products
Introduction : ensuring the integrity of therapeutic peptides
In the growing field of biopharmaceuticals, peptides represent a class of active molecules with complex structural and functional characteristics.
Their therapeutic potential is immense, but it also comes with analytical challenges. FILAB Laboratory offers specialized peptide analysis and characterization services tailored to support every stage of drug development and quality control. Our scientific approach ensures a precise understanding of each peptide’s composition, stability, and impurities—key factors in ensuring product safety and regulatory compliance.
Peptides offer great therapeutic potential, but their development presents challenges due to their complexity and varied physicochemical properties. This complexity requires a multitude of analytical techniques to meet strict regulatory requirements.
Characterization and comparative study
Impurity analysis of raw materials and finished products...
Analytical method transfer and validation
Stability studies
Peptide mapping to establish the profile of sequences.
Mass spectrometry-based peptide identification for the analysis of modifications and impurities.
Conformity verification by LC-MS/MS
FILAB offers peptide characterization and peptide mapping services
Our analytical approach to peptide characterization
At FILAB, we rely on a rigorous scientific methodology to provide reliable, detailed insights into the structure and behavior of peptides.
Peptide mapping is one of the central techniques we employ. This process involves fragmenting the peptide chain enzymatically and analyzing the resulting sequences to confirm the primary structure. This step is critical for verifying the identity of therapeutic peptides and detecting sequence errors or unexpected modifications.
Peptide mapping
This technique ensures that the amino acid sequence of a protein corresponds to the expected sequence.
Modifications occurring after protein translation, such as glycosylations or oxidations, can be identified.
Peptide mapping can be used to check protein integrity and detect unwanted fragments or degradation.
This technique is essential for ensuring the quality of biopharmaceutical products before they reach the market, guaranteeing that the protein structure is correct and compliant with regulatory requirements.
Which industrial sectors use peptides?
Peptides are versatile molecules used in a variety of sectors, from pharmaceuticals to cosmetics… Their specificity and efficacy enable them to play a key role in developing new treatments or improving skin care. This diversity of applications underlines the considerable potential of peptides and the importance of continuing research in this field.
our ressources
Our biopharmaceutical services
Advanced techniques and equipment
The analytical techniques used by FILAB include high-resolution liquid chromatography–mass spectrometry (LC-MS). These instruments enable us to provide both qualitative and quantitative data with high accuracy and sensitivity. Our methods comply with international guidelines, including ICH Q6B, making them suitable for regulatory documentation and GMP environments.
Our laboratory is also equipped to conduct stress testing and stability studies. These assessments simulate long-term storage conditions or accelerated degradation to understand how peptides behave over time. We then evaluate the resulting degradation pathways and generate data that supports shelf-life justification or formulation adaptation.
Impurity profiling and regulatory compliance
Another critical aspect of our service is impurity profiling. During synthesis, storage, or formulation, peptides may be subject to contamination or chemical alteration.
FILAB’s analytical protocols allow us to identify and quantify these impurities, whether they are residual solvents, synthesis byproducts, or degradation compounds. This helps our clients maintain compliance with strict biopharmaceutical standards and minimize the risk of regulatory rejection.
Our reports provide clear, structured findings that include method details, raw data, and expert interpretation. This level of documentation is particularly valuable when submitting data to health authorities or preparing for audits.
A dedicated partner for biopharmaceutical projects
FILAB is more than a service provider—we are a scientific partner. Our team of PhD-level chemists and biopharmaceutical analysts works closely with clients to design custom testing protocols and provide technical guidance at every stage of the project. We understand the constraints of clinical development timelines and adapt our services to deliver fast, high-quality results.
From early-stage R&D to final batch release testing, our experience in biotherapeutic peptide characterization is a valuable asset. We help clients bring safe, effective, and well-characterized peptide products to market with confidence.
FAQ
A peptide is a polymer of amino acids linked together by peptide bonds. There is a wide variety of peptides. A peptide can be considered a small protein, or rather, a segment of a protein, as proteins are made up of one or more long chains of peptides. The main distinction between a peptide and a protein lies in their size. FILAB carries out peptide analyses.
When you choose FILAB, you benefit from :
- Recognized expertise in chemical and biological analysis.
- State-of-the-art techniques for accurate, reliable results.
- Expert interpretation of results for solutions tailored to your needs.
Peptide mapping is an analytical technique used primarily for protein characterization, particularly in biopharmaceutical product development. The method involves fragmenting a protein into several smaller peptides via enzymatic digestion. Once fragmented, these peptides are separated and analyzed using techniques such as liquid chromatography-mass spectrometry (LC-MS).
Peptide mapping is an analytical technique that breaks down a peptide or protein into smaller fragments to verify its amino acid sequence and detect structural variations.
It is essential for confirming the identity, purity, and consistency of biotherapeutic peptides, especially during quality control and regulatory submission phases.
FILAB uses high-resolution techniques such as liquid chromatography–mass spectrometry (LC-MS) and HPLC to conduct in-depth peptide characterization.
These methods allow for sequence confirmation, impurity profiling, detection of post-translational modifications (PTMs), and evaluation of stability under stress conditions.
Yes, FILAB specializes in identifying PTMs.
These modifications can significantly affect the function and stability of therapeutic peptides and must be monitored closely to ensure product efficacy and safety.
While FILAB is not a GMP-certified laboratory, our methods and reporting align with international standards such as ICH Q6B.
We regularly support clients with regulatory submissions by providing validated methods, stability data, and impurity profiles that meet EMA and FDA expectations.
Absolutely. FILAB conducts peptide stability studies under ICH-recommended conditions, as well as accelerated degradation studies.
This allows clients to understand the shelf life and degradation pathways of their peptides, which is essential for product development and packaging decisions.
To begin a project, you can contact our team via our online form or by phone. We’ll provide instructions for safe sample shipment, discuss your objectives, and define a customized testing protocol tailored to your peptide and regulatory context.
Turnaround time depends on the complexity of the analysis and the specific tests required. We invite you to contact us directly if you need peptide mapping.
