Laboratory analysis using High Performance Liquid Chromatography (HPLC)
Your needs: analyzing active substances or impurities in your products by High-Performance Liquid Chromatography (LC) and (HPLC)
What is HPLC analysis?
La chromatographie en phase liquide à haute performance (HPLC) permet d’identifier et de quantifier des substances organiques (principes actifs ou contaminants) dans diverses matrices, parfois complexes : produits cosmétiques, produits pharmaceutiques, ingrédients naturels, matrices biologiques…
Une analyse HPLC par chromatographie en phase liquide (HPLC) est une technique analytique utilisée pour séparer, identifier et quantifier des composés dans une solution. En somme, cette méthode permet de dissocier les molécules présentes en fonction de leurs caractéristiques physico-chimiques.
Why carry out an analysis by Liquid Chromatography (LC)?
Liquid chromatography (LC) is an analytical technique widely used in chemistry laboratories for samples containing several compounds, such as plant extracts or biological samples, but also for the identification of contaminants or impurities.
LC can indeed be used to analyze impurities in pharmaceutical products or contaminants in drinking water, for example. The results obtained through liquid chromatography are highly accurate and reliable, making it a preferred technique.
Our services for HPLC analysis in the laboratory
The FILAB laboratory offers comprehensive services for high-performance liquid chromatography (HPLC) analysis. Our HPLC analysis are carried out with precision and rigor, and we work closely with our clients to adapt our analytical methods to their specific needs.
Analysis of active ingredients
Quantification of regulated substances: nitrosamines, bisphenol, aldehydes,...
Quantification of sugars
Identification of substances within the framework of REACH
Identification of impurities
Development and validation of analytical methods
Identification of ghost peaks
Polymer de-formulation and identification of additives (antioxidants, UV stabilizers...)
Our solutions: analysis by high-performance liquid chromatography (HPLC)
The expertise of our highly qualified teams enables us to offer you our know-how in the analysis of your materials by high-performance liquid chromatography. Our specialists support you from addressing your issue through to interpreting the results, in order to provide you with lasting corrective solutions.
The benefits of an HPLC analysis carried out in the laboratory
The benefits of an HPLC analysis carried out in the laboratory are numerous. First of all, this technique offers great precision and sensitivity in molecule identification. It also provides great flexibility in terms of sample types and compounds tested. In addition, laboratory HPLC analysis is often complemented by additional detection techniques, such as mass spectrometry, for more in-depth analysis. Finally, this technique is particularly useful in the pharmaceutical and food industries to ensure product quality, safety, and compliance.
Examples of materials subjected to HPLC analysis
The materials subjected to HPLC analysis are extremely varied, ranging from organic compounds to polymers and metals. Natural substances, such as amino acids, proteins, and sugars, are often examined by high-performance liquid chromatography. Medicines are also analyzed by HPLC, as are impurities in pharmaceutical products.
In short, HPLC is a key analytical method for efficiently examining a wide range of materials and substances.
Our Liquid Chromatography (LC) analysis by industry sector…
Industry sectors
Our detectors: precise technical solutions tailored to your industry
FILAB has a range of detectors suited to high-performance liquid chromatographic analysis (HPLC). The choice of detector varies depending on the nature of the samples, the objectives of the analysis, and the required sensitivity.
Among the HPLC detectors commonly used:
FAQ
There are two commonly used types of chromatography:
- liquid chromatography (LC)
- high-performance liquid chromatography (HPLC).
These two techniques differ in the characteristics of their stationary and mobile phases. Indeed, HPLC uses finer stationary phases and high-flow pumps for better molecular resolution, whereas conventional liquid chromatography can be performed at a constant flow rate and with less refined stationary phases. In all cases, the goal is to achieve a clear separation of the sample components, allowing precise analysis of each substance present. Ultimately, this results in higher resolution and faster separation of the components in a sample with HPLC.
Physicochemical properties such as acidity, basicity, polarity, solubility, and molecular size are important for the separation of compounds, so they are essential in HPLC. Indeed, these properties help determine the behavior of compounds on the chromatographic column, and therefore the identification of the sample components.
HPLC applies to:
Aqueous or organic solutions
Raw materials
Finished products (creams, tablets, liquids…)
Synthesis intermediates
Complex samples (biological, environmental…)
The filab laboratory offers HPLC method development and validation according to the specific requirements of your product, in compliance with ICH guidelines, Pharmacopoeia, etc.
Thanks to optimized methods and high-performance detectors (e.g. HPLC-MS), it is possible to quantify impurities down to a few ppm, or even ng/mL in some cases.
