Laboratory for analysis and measurement of circular dichroism (CD)

Chemical analysis Problem solving
More than 140 people
More than 140 people at your service
5200 m² laboratory
5200 m² laboratory + 99% of services are provided in-house
Accredited laboratory
Accredited laboratory COFRAC ISO 17025

You wish to perform the analysis and measurement of circular dichroism (CD)

Structural analysis of biomolecules and chiral materials

FILAB laboratory, an expert in biopharmaceutical analysis, offers its clients its expertise in circular dichroism (CD) measurement to characterize the secondary structure of proteins and peptides, monitor the conformation of biotherapies, control the chirality of materials and evaluate the stability of your formulations under near-real conditions.

Our services and study formats for Circular Dichroism (CD)

Standard characterization (far UV CD)

Single spectrum or multiple repetitions
Deconvolution (α helices / β sheets / coil)
Summary report
Comparability study

Comparison between batches/formulations
Similarity indicators (Δ ellipticity, spectral overlap, internal metrics)
Formulation/Stability Recommendations
Thermal stability and/or pH

Temperature ramp (10→95°C) with monitoring of the Tm
Effect of pH, salts and excipients
Reversibility study (cooling)
Interaction & screening

Spectra before/after ligand (small molecule, ion, cofactor)
Short kinetic energy if necessary
Analysis of conformational changes

Our complementary analytical techniques

FTIR

To confirm the secondary structure

DSC

To measure thermal transitions

SEC

To evaluate molar mass and aggregation

LC-MS

To analyze the integrity and composition of biomolecules

The FILAB laboratory performs the analysis and measurement of circular dichroism (CD)

Why perform a Circular Dichroism (CD) analysis?

The CD is an essential tool for:

Study the secondary structure (α helices, β sheets, disordered conformations)

Compare batches, formulations, or storage conditions

Checking structural integrity after a process change

Checking the conformational stability of a protein

Follow a thermal or chemical denaturation

Detecting subtle differences between two versions of the same biomaterial (biosimilars, variants, mutations…)

What FILAB offers you for your Circular Dichroism measurements CD

Expert support: assistance with experimental design (spectral ranges, temperature, buffer, concentration)

Stability studies

Fast and robust measurements: spectra in the far UV (190–260 nm) and/or near UV (260–320 nm)

Confidentiality and quality: project management, traceability, and services under standards adapted to your sector

FAQ

Does Circular Dichroism (CD) replace DSC or NMR?

No. Circular Dichroism CD is fast and sensitive to variations in secondary structure; it is complementary to DSC (thermodynamics) and NMR (fine structure, local interactions).

How far can we go below 200nm?

This depends on the transparency of the buffer and the optical path. We optimize the conditions to maximize the useful information..

Can studies be conducted under real-world conditions (salt, excipients)?

Yes, subject to optical compatibility. We offer alternative formulations when necessary.

What are the areas of application of Circular Dichroism (CD)?
  • Biopharmaceuticals: antibodies, fragments, therapeutic peptides, enzymes, vaccines, biosimilars
  • Biotechnology and diagnostics: recombinant proteins, media, reagents
  • Cosmetics and dermo-cosmetics: functional peptides, skincare proteins, formulation stability
The filab advantages
A highly qualified team
A highly qualified team
Responsiveness in responding to and processing requests
Responsiveness in responding to and processing requests
A COFRAC ISO 17025 accredited laboratory
A COFRAC ISO 17025 accredited laboratory
(Staves available on www.cofrac.com - Accreditation number: 1-1793)
A complete analytical park of 5,200m²
A complete analytical park of 5,200m²
Tailor-made support
Tailor-made support
Video debriefing available with the expert
Video debriefing available with the expert
Anaïs DECAUX Customer Support Manager
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