Objectives
Understand the operating principles of LC/MSMS and LC/MS
Meet increasingly stringent regulatory requirements and constraints
Gain theoretical and practical skills on this topic
Learn how to acquire and reprocess data
Teaching methods
- Projection of the training materials
- Distribution of the training materials
- Training led by experts in the requested techniques
Target audience
Technicians or engineers in charge of research & innovation, process & industrialization, production & maintenance…
Program
For more information about the program, please contact us
Information updated on 10.10.2024
Practical information
- In-company training
- Questionnaire at the start and end of the training (quiz)
- Attendance sheet
- Duration: 2 days
- Accessibility for people with reduced mobility and disabilities
- Adaptable according to the client’s needs
- Price on request
- No prerequisites required to take this training
- A certificate of completion will be issued to participants at the end of the training
Our training courses are accessible to people with disabilities. We will offer adaptation solutions (for example: training materials, training pace, educational support, etc.) and adjustments according to your needs.
Does your team need training in LC/MSMS and LC/MS techniques...
What is MS mass spectrometry?
Mass spectrometry, thanks to the technical advances it has undergone in recent years, is a method often used to meet these precision and sensitivity criteria. It provides both quantitative data (content of elements) and qualitative data (nature), making it possible to reduce overall analysis times.
Combined with LC (or HPLC), MS offers better detection quality and can analyze unknown peaks or degradation products at very low concentration and of any type (organic or inorganic).
At FILAB, we have dual MS/MS detection, allowing us to analyze very low concentrations.
Why train in LC-MSMS and LC-MS?
The applications of LC/MS/MS and LC/MS analysis are numerous. Thanks to this training, you will be able to:
Carry out accurate quantitative and qualitative analysis to identify and quantify compounds in complex matrices.
Detect and characterize contaminants, impurities or trace substances in various sectors (pharmaceutical, agri-food, environmental, etc.).
Anticipate and resolve technical anomalies to maximize the efficiency and performance of your equipment.
Optimize the development of analytical methods tailored to your laboratory's requirements.
Improve the sensitivity and specificity of your analysis for reliable and reproducible results.
Training in LC-MS/MS and LC-MS means acquiring the essential skills to make full use of this technology in the laboratory.
In what context should you take an LC MS training course?
Liquid chromatography coupled with mass spectrometry (LC/MS) is a widely used technique in physico-chemical analysis and bioanalysis. Thanks to technological advances, its use has expanded to many applications, for both quantitative analysis (assay) and qualitative analysis (structural identification).
However, fully harnessing the potential of LC/MS(/MS) requires a solid theoretical understanding and in-depth mastery of its fundamental mechanisms. The training provided by FILAB aims to give you the essential knowledge to get started with or improve your LC/MS or LC-MS/MS analysis.
Why choose FILAB for any training in analytical chemistry or materials?
The FILAB laboratory is QUALIOPI certified for its training activities.
Thanks to this certification, the training courses we provide may be eligible for funding requests from OPCOs.
Analytical training is essential for professionals looking to master analysis and characterization techniques; in fact, it covers a wide range of techniques intended for the analysis and characterization of substances, materials, or products across various industrial sectors.
Our analytical training programs are designed to equip participants with the skills needed to excel in their field, combining rigorous theory with practical applications. Discover how our analytical training can transform your skill set within your company.
Several reasons explain the growing number of training requests received by FILAB:
Tailor-made technical training: most training courses offered by many training providers are standard, not tailor-made, and do not address your technical challenges
Highly qualified people: our trainers are laboratory professionals, PhDs, or specialized engineers
Industry knowledge: FILAB has been delivering training to its clients for over 10 years, whatever the industrial sector
A unique profile with dual expertise in Chemistry and Materials
A human-scale organization, which allows us to be more responsive and more flexible
Our FAQ
This training aims to provide an in-depth understanding of how LC-MS/MS and LC-MS work, while ensuring mastery of the constantly evolving regulatory requirements. It provides the essential theoretical and practical skills needed to optimize the use of these analytical techniques. Participants will also learn how to perform data acquisition and reprocessing, ensuring reliable analysis that comply with quality standards.
It is intended for engineers and laboratory technicians who are new to LC/MS and want to master the fundamentals and improve their practice.
At the end of the training, participants will be able to:
- Understand the theoretical and practical principles of LC/MS.
- Develop analytical methods tailored to the laboratory's needs.
- Identify and correct common issues to ensure reliable and reproducible analysis.
No, this training is also designed for beginners in LC/MS. A general knowledge of analytical techniques is a plus, but not mandatory. In addition, our training courses can be adapted to your needs.
It combines both aspects:
Theory to understand the fundamental principles.
Practice with case studies and concrete solutions to optimize your analysis.
By mastering LC/MS best practices, participants will be able to:
- Develop reliable and reproducible methods.
- Minimize errors and time loss related to technical anomalies.
- Increase the accuracy and speed of analysis.
Liquid chromatography coupled with mass spectrometry (LC-MS) and its tandem version (LC-MS/MS) can be referred to by several other names depending on the context and field of application:
🔹 HPLC-MS (High-Performance Liquid Chromatography-Mass Spectrometry) – Used when high-performance liquid chromatography is specified.
🔹 LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry) – Also called MS², referring to tandem analysis for greater selectivity and sensitivity.
🔹 TOF-SIMS (Time-of-Flight Mass Spectrometry) – When a Time of Flight mass analyzer is used.
🔹 HRMS (High-Resolution Mass Spectrometry) – When high-resolution mass spectrometry is used, often in combination with LC.
🔹 QTOF-MS (Quadrupole Time-of-Flight Mass Spectrometry) – Referring to a configuration in which a quadrupole is coupled to a TOF analyzer.
These different names vary depending on the equipment and specific applications (pharmaceutical, environmental, agri-food, bioanalysis, etc.).
To go further
Sensitivity optimization relies on several factors:
✔ Choice of mobile phase: Use volatile buffers such as ammonium acetate or ammonium formate to avoid ion suppression.
✔ Optimization of source parameters: Adjust the desolvation temperature, capillary voltage, and nebulization pressure to maximize ionization.
✔ Adjustment of the ionization mode (ESI/APCI): Select the appropriate ionization source according to the polarity and volatility of the analytes.
✔ Optimization of the signal-to-noise ratio: Work on sample dilution and the removal of matrix interferences via SPE or isotopic dilution.
Matrix effects, which can lead to signal suppression or enhancement, can be reduced by:
✔ Using isotopically labeled internal standards to compensate for ionization variations. Matrix effects, which can lead to signal suppression or enhancement, can be reduced ns.
✔ Optimizing sample preparation: solid-phase extraction (SPE) or effective filtration.
✔ Modifying the mobile phase composition: buffering and adjusting the pH to improve separation.
✔ Working on sample dilution to reduce the effects of impurity co-elution.
The choice of fragmentation mode (CID – Collision-Induced Dissociation, HCD – Higher-energy Collisional Dissociation) directly affects:
✔ Fragment specificity: Some modes generate more specific ions for identification.
✔ Sensitivity: Some fragments have a better response depending on the collision energy applied.
✔ Resolution: A setting that is too high can reduce ion transmission and decrease sensitivity.
In the pharmaceutical industry, LC-MS/MS ensures quality control by enabling:
✔ Accurate quantification of active ingredients and detection of impurities (ICH Q3A/B standards).
✔ Analysis of degradation products to ensure drug stability.
✔ Bioanalysis in pharmacokinetics, essential for bioavailability studies.
