Biopharmaceutical manufacturers, are you looking to carry out fluorimeter analysis?
La fluorimétrie est une technique d’analyse optique basée sur la capacité de certaines molécules à émettre de la lumière après excitation. Elle est particulièrement utilisée en biopharmaceutique pour sa sensibilité et sa sélectivité.
Fluorimeter analysis: a key technique for the biopharmaceutical industry
Fluorimeter analysis is a reference technique for the detection, quantification, and monitoring of fluorescent compounds at very low concentrations. In thebiopharmaceutical industry, it plays a central role in securing the development, production, and quality control of biomolecules, proteins, peptides, nucleic acids or contaminants.
At the FILAB laboratory, this technique is carried out in a controlled environment, using high-sensitivity equipment and expertise tailored to the regulatory and industrial constraints of the biopharmaceutical sector and to theanalysis of biomedicines.
The applications of fluorimetry analysis in biopharmaceuticals
Fluorimeter analysis is used at different stages of the biopharmaceutical product lifecycle:
Protein and biomolecule quantification (BSA, antibodies, enzymes, peptides)
Purity control and detection of fluorescent impurities
Stability and degradation
Bioproduction process monitoring (fermentation, purification, formulation)
Residual RNA quantification
Production quality control
The FILAB laboratory supports manufacturers in fluorimeter analysis
When should you call on a service laboratory specialized in fluorimeter analysis?
Industrial companies call on the services of the FILAB laboratory, which has this technique, notably to:
meet a regulatory or quality requirement
investigate a non-conforming result
compare batches or formulations
secure a analytical development
outsource a sensitive analysis not carried out in-house
Why use fluorimetry in biopharmaceuticals?
La fluorimétrie repose sur la mesure de l’émission lumineuse d’une molécule après excitation. Elle se distingue par :
une excellente sensibilité, souvent supérieure à l’absorbance UV-visible
une forte sélectivité, grâce au choix précis des longueurs d’onde
une détection de traces, compatible avec des matrices complexes
Ces caractéristiques en font une technique particulièrement adaptée aux exigences de fiabilité, de reproductibilité et de traçabilité propres au secteur biopharmaceutique.
Our technical resources for biopharmaceutical analysis
FAQ
Yes. Thanks to its high selectivity and the precise choice of excitation and emission wavelengths, fluorimetry makes it possible to analyze complex matrices typical of the biopharmaceutical sector (culture media, formulations, excipients, buffers).
UV-Vis measures light absorption, while fluorimetry measures light emission. This difference enables fluorimetry to deliver greater sensitivity, lower detection limits, and a better signal-to-noise ratio.
Yes. Labeling agents or fluorescent probes can be used to make non-naturally fluorescent molecules detectable, such as certain proteins, peptides, DNA, or contaminants.
Yes. analysis can be carried out using validated methods, with full traceability of the tests, meeting the quality and regulatory requirements of the biopharmaceutical sector.
